Hi miroma
This has been a constant problem for us especially when rotating pages in Impose. The preview in Impose shows no gutter. The preview from the Hold queue shows no gutter, yet the file prints with a gutter.
The only solution I have found for this is to save the job as a flattened file from Impose.
Konica Minolta bizhubpress C1085 - Fiery System FS150 Pro
Fiery IC-308 v2.0 - CWS 5.8.0.39 SP2 on Windows 7 Pro SP1
Konica Minolta bizhub Pro C6501 (65C-KM V 3.0) - Fiery System 9
Xerox DC250 (DC250 V 1.2) - Fiery System 8e Release 2
Fiery Pro80 65-55C-KM PS Color Server 3.0a eu - CWS 5 (5.7.0.29) on Windows XP SP2
EFI CWS 6.1 (6.1.0.28) on Dell Studio XPS running Windows 7 Pro
AMD Athlon II X4 640 3.00GHz 4Gb RAM